Ubuchwepheshe bokuthola i-Enzvme be-Enterococcus Okokuhlola amanzi Isithombe Esifakiwe

Ubuchwepheshe bokubona i-Enzvme be-Enterococcus Ukuhlola amanzi

Ikhodi Yomkhiqizo:

Igama lento:I-Enzvme ukuthola i-technolouv ye-Enterococcu

Umlingiswa: Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ezikhanyayo Ukucaca

Okungenambala:noma okuphuzi okukhanyayo

pH 7.0 - 7.6

Isisindo: 2.7 士 0.5g

Isitoreji: Isitoreji ku-4°C - 8°C, Endaweni epholile eyomile futhi ivikele ekukhanyeni

Ukufaneleka: Unyaka ongu-1, Bona ukupakishwa kwe-reagent yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.

Isayensi:Engeza isampula yamanzi aqukethe amagciwane e-Enterococcus, yenza amagciwane okuqondiswe kuwo ku-Mug medium at 41°C 土 0.5°C, kanye nama-enzyme athile e-ological akhiqizwa amagciwane e-Enterococcus(3 -0 -gluco sidase angabola

I-fluorescent substrate inkomishi endaweni yekomishi ezokhiqizwa (3 -D-glucoside ((3 -0 -glucoside) kanye

umkhiqizo we-fluorescent ongu-4-methyl umbelferone.Bheka i-fluorescence kusibani se-UV esingu-366nm, ubale ngediski yokuthola inani, bese ubuza ithebula le-MPN ukuze ubale imiphumela.

Iphakheji: 100 - iphakethe lokuhlola

Thumela i-imeyili kithi IKHATHALOGU

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Umlingiswa	Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ngokukhanyayo
Ukucaca	Okungenambala noma okuphuzi okukhanyayo
PH	7.0 - 7.6
Wayisishiyagalombili	2.7 士 0.5g
Isitoreji	ku-4°C - 8°C, endaweni epholile eyomile futhi uvikele ekukhanyeni
Ukuba semthethweni	Unyaka ongu-1, Bona ukupakishwa kwe-reagent yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.
Isayensi	Engeza isampula yamanzi aqukethe amagciwane e-Enterococcus, yenza amagciwane okuqondiwe ku-Mug medium at 41°C 土 0.5°C, kanye nama-enzyme athile egazi akhiqizwe amagciwane e-Enterococcus(β-D-glucosidase angabola inkomishi ye-fluorescent substrate inkomishi ukuze ikhiqize. (β-D-glucoside ((β-D-glucoside) kanye nesici somkhiqizo we-fluorescent 4-methyl umbelliferone. Qaphela i-fluorescence kusibani se-UV esingu-366nm, bala ngediski yokuthola inani, bese ubuza ithebula le-MPN ukuze ubale imiphumela.
Iphakheji	100 - iphakethe lokuhlola

Indlela yokusebenzisa Ukuhlolwa kwekhwalithi

I-Setp1:Thatha isampula lamanzi elingu-100ml nebhodlela lesampula eliyinyumba elingu-100ml/ibhodlela lobuningi, engeza into ephindayo, ncibilikisa, nesiko amahora angama-24 ku-41°C 士 0 .5°C.

I-Set2:Isinqumo somphumela

I-Non fluorescence = Imiphumela engemihle I-fluorescence eluhlaza okwesibhakabhaka = Imiphumela emihle

Ukutholwa komthamo

1.Sebenzisa ibhodlela lesampula eliyinyumba elingu-100ml ukukala isampula lamanzi elingu-100ml.

2.Engeza i-reagent, yiqede, uyithele ku-51 Quanti Tray noma 97 Quanti-Tray.

3.Pakisha i-Quanti-Tray ngomshini olawulwayo wokuvala umthamo, futhi uyifukamele ku-41°C 士 0 .5 ° C amahora angu-24.

4.Bheka esivivinyweni sekhwalithi ukuze uthole incazelo yemiphumela, bese ubheka ithebula le-MPN ukuze ubale.

Okwedlule: I-Coliform Group Enzvme i-substrate yokuthola i-reagent yokuhlola amanzi

Olandelayo: I-Intelligent colony analyzer yokuhlola amanzi

Bhala umyalezo wakho lapha futhi usithumelele wona