I-Coliform Group Enzvme i-substrate yokuthola i-reagent yokuhlola amanzi

Ikhodi Yomkhiqizo:

Igama Lento : I-Cotiform Group Enzvme ukuthola i-substrate reagent

Umlingiswa: Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ezikhanyayo

Ukucaciswa : Idigri Elingenambala noma eliphuzi kancane

PH 7.0-7.8

Isisindo: 2.7士 0.5 g

Isitoreji: Ukugcina isikhathi eside, ukomisa, ukuvala nokugwema ukugcinwa kokukhanya ku-4°C – 8°C

Isikhathi sokusebenza : unyaka ongu-1

Thumela i-imeyili kithi IKHATHALOGU

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Isimiso Sokusebenza

Umlingiswa	Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ngokukhanyayo
Idigri yokucacisa	Engenambala noma ephuzi kancane
PH	7.0-7.8
Wayisishiyagalombili	2.7士 0.5 g
Isitoreji	Ukugcina isikhathi eside, ukomisa, ukuvala nokugwema ukugcina ukukhanya ku-4°C - 8°C
Isikhathi Sokusebenza	1 iminyaka
Working Isimiso	Kumasampula amanzi aqukethe inani lamabhaktheriya e-coliform, amabhaktheriya okuhlosiwe akhuliswe ku-ONPG-MUG medium ngo-36 土1C. I-enzyme ethize ye-betagalactosidase ekhiqizwe ingqikithi yebhaktheriya ye-coliform ingabola i-substrate yomthombo wombala we-ONPG-MUG medium, eyenza isiko eliphuzi;Okwamanje, i-Escherichia coli ikhiqiza i-beta-glucuronase ethile ukuze ibolise i-substrate ye-fluorescent MUG ku-ONPG-MUG medium futhi ikhiqize isici se-fluorescence.Isimiso esifanayo, iqembu le-coliform lokubekezelela ukushisa (iqembu le-fecal coliform) lizobolisa i-substrate yomthombo wombala ONPG ku-ONPG-MUG medium ku-44.5 土 0.5 °C, okwenza okumaphakathi kubephuzi
Ukucaciswa kwephakheji	200/ibhokisi

Indlela yokusebenzisa Ukutholwa kwekhwalithi

Isinyathelo 1: Faka i-reagent kusampula yamanzi ayi-100ml, uyiqede.Namasiko 24h ngo-36 士 1 ° C
isinyathelo2:
Funda umphumela Ongenambala = negethivu

Okuphuzi = i-coliform iphelele

Ophuzi + fluorescence = Escherichia coli positive

Qaphela: Iqembu le-coliform elibekezelela ukushisa (i-fecal coliform) lidinga ukukhuliswa ku-44.5 士 0 .5°C 24h, futhi okuphuzi kuphozithivu.

Ukutholwa komthamo

1.Engeza i-reagent kusampula yamanzi angu-100ml, yiqede.
2.Thela kupuleti lokubona izimbobo ezingu-51 noma ezingu-97

3.Sebenzisa i-LK sealer ukuze uvale ipuleti lokubona, futhi uhlakulele u-24h ku-36 °C.

4.Ukuhlolwa kwekhwalithi yemiphumela kwasetshenziswa ukuhlola ukubalwa kwetafula le-MPN.

Qaphela: Iqembu le-coliform elibekezelela ukushisa (iqembu le-fecal coliform) lidinga ukukhuliswa kokuthi 44.5 士 0.5°C 24h, futhi imbobo Ephuzi iphozithivu, futhi isibalo sethebula le-MPN esihambisanayo siyatholwa.

Okwedlule: Ibhodlela lesampula eliyinyumba elingu-100ml / ibhodlela lenani Lokuhlolwa kwamanzi

Olandelayo: Ubuchwepheshe bokubona i-Enzvme be-Enterococcus Ukuhlola amanzi

Bhala umyalezo wakho lapha futhi usithumelele wona