I-Enzvme discovery technolouv ye-Enterococcus Yokuhlola amanzi

Igama lento ;Enzvme ukuthola technolouv of Enterococcu

Umlingiswa Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ngokukhanyayo Ukucaca

Okungenambala noma okuphuzi okukhanyayo

pH 7.0 - 7.6

Isisindo 2.7 士 0.5g

Isitoreji Isitoreji ku-4°C – 8°C, Endaweni epholile eyomile futhi uvikele ekukhanyeni

Ukufaneleka Unyaka O-1, Bona ukupakishwa kwe-reagent yedethi yokukhiqiza kanye nosuku lokuphelelwa yisikhathi.

Isayensi

Engeza isampula yamanzi aqukethe amagciwane e-Enterococcus, yenza amagciwane okuhlosiwe endaweni ye-Mug ku-41°C 土 0.5°C, kanye nama-enzyme athile e-ological akhiqizwa amagciwane e-Enterococcus (3 -0 -gluco sidase angabola

I-fluorescent substrate inkomishi endaweni yekomishi ezokhiqizwa (3 -D-glucoside ((3 -0 -glucoside) kanye

umkhiqizo we-fluorescent ongu-4-methyl umbelferone.Bheka i-fluorescence kusibani se-UV esingu-366nm, ubale ngediski yokuthola inani, bese ubuza ithebula le-MPN ukuze ubale imiphumela.

Iphakheji 100 - iphakethe lokuhlola