Igama Lento :I-Cotiform Group Enzvme i-reagent yokuthola indawo engaphansi
Uhlamvu Lo mkhiqizo uyizinhlayiya ezimhlophe noma eziphuzi ngokukhanyayo
Idigri yokucacisa engenambala noma ephuzi kancane
PH 7.0-7.8
Isisindo 2.7士 0.5 g
Isitoreji: Ukugcina isikhathi eside, ukomisa, ukuvala nokugwema ukugcinwa kokukhanya ku-4°C – 8°C
Isikhathi Sokusebenza Unyaka ongu-1
Isimiso Sokusebenza
Emasampula amanzi aqukethe amabhaktheriya e-coliform ephelele, amabhaktheriya ahlosiwe akhuliswe ku-ONPG-MUG medium ku-36 土 1 C. I-enzyme ethize ye-betagalactosidase ekhiqizwa amabhaktheriya aphelele e-coliform ingabola i-substrate yomthombo wombala we-ONPG-MUG medium, okwenza isiko siphuzile; Okwamanje, i-Escherichia coli ikhiqiza i-beta-glucuronase ethile ukuze ibolise i-substrate ye-fluorescent MUG ku-ONPG-MUG medium futhi ikhiqize isici se-fluorescence. Umgomo ofanayo, iqembu le-coliform elibekezelela ukushisa (iqembu le-fecal coliform) lizobolisa umthombo wombala ongaphansi we-ONPG ku-ONPG-MUG medium at
44.5 imizuzwana 0. 5 °C, okwenza okumaphakathi kubephuzi
