Imikhiqizo
Ikhithi ye-Elisa antibody engaqondile ye-Brucellosis
Ikhithi ye-Elisa antibody engaqondile ye-Brucellosis
| Isifinyezo | Ukutholwa kwe-Antibody ethize Yezinyawo Nezifo Zomlomo I-BRU |
| Isimiso | Umgomo we-Test Brucellosis(Bru) antibody Elisa test kit ingasetshenziswa ukuthola i-Brucellosis antibody ku-serum noma ku-plasma yeBovine and Pig. |
| Okuhloswe ngakho Ukuthola | I-antibody ye-BRU |
| Isampula | I-Serum |
| Ubuningi | 1 kit = 192 Test |
| Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
|
Ulwazi
I-Brucellosisiyathelelana kakhului-zoonosisokubangelwa ukungenwa yi-unpasteurizedubisinoma kungaphekwa kahleinyamaezilwaneni ezithelelekile, noma ukuthintana eduze nokuphuma kwazo.
Yaziwa nangokuthi i-undulant fever, i-Malta fever, kanye ne-Mediterranean fever.
Isimiso Sokuhlolwa
Lokhu ikhithi sebenzisa ngokungaqondile ELISA indlela, okuhlanzekile Brui-antigen is efakwe ngaphambili on i-enzyme kahle kahle imichilo. Uma uhlola, engeza buhlanjululiwe i-serum isampula, ngemva ukufukamela, if Lapho is Bru ethize amasosha omzimba, it intando hlanganisa nge i efakwe ngaphambili i-antigen, lahla i engahlanganisiwe amasosha omzimba futhi okunye izingxenye nge ukuwasha; bese engeza i-enzyme hlanganisa, lahla i engahlanganisiwe i-enzyme hlanganisa nge
ukuwasha. Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis iqondile ingxenye yokuqukethwe kwamasosha omzimba kusampula.
Okuqukethwe
| I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
| 1 |
| 1e/2e | |
| 2 |
| 2ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2 eya/4 | |
| 10 | i-serum dilution microplate | 1e/2e | |
| 11 | Isiyalezo | 1 ama-pcs |
