I-Brucellosis Antibody Competitive Elisa Kit

I-BRU amasosha omzimbaELISAikhithi yokuhlolajwayele ukutholakala kwama-antibodies e-Brucellosis ku-serum yezingulube, izinkomo,sheep kanye nembuzi .

Sebenzisa le kit indlela ye-ELISA yokuncintisana ngaphambi kokugqokai-BRU ama-antigens emithonjeni ye-microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululiwe futhii-enzyme ebizwa ngokuthi i-anti-I-BRU I-monoclonal antibody, ngemva kokufukamela, uma ikhona babe I-BRU i-antibody, izohlangana ne-antigen eboshwe ngaphambili, i-antibody ekusampula block inhlanganisela ye-monoclonal antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha;Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula..