Imikhiqizo
I-Avian Leukemia P27 Antigen ELISA Kit
I-Hydatid Disease Infection Antibody ELISA Kit
| Isifinyezo | esetshenziselwa ukuthola i-Avian leukosis P27 antigen egazini lezinyoni, indle, i-cloaca, nokumhlophe kweqanda. |
| Isimiso | I-Avian Leukosis (AL) P27 antigen Elisa kit isetshenziselwa ukuthola i-Avian leukosis P27 antigen egazini lezinyoni, indle, i-cloaca, kanye nokumhlophe kweqanda.
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| Okuhloswe ngakho Ukuthola | I-Avian Leukosis (AL) i-antigen ye-P27 |
| Isampula | I-Serum
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| Ubuningi | 1 kit = 192 Test |
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Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃. Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12. Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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Ulwazi
I-Avian Leukosis (AL) igama elihlangene lezifo ezihlukahlukene ezihlobene nesimila ezinkukhuni ezibangelwa i-Avian Leukosis Virus (ALV) emndenini we-Retroviridae. Lesi sifo sisatshalaliswa emhlabeni wonke futhi sinezinga eliphezulu lokutheleleka. Kungabangela ukufa kanye nokuwohloka kwezinkukhu, kunciphise amandla okukhiqiza umhlambi, futhi kungenye yezifo eziyinhloko ezibeka engcupheni ukuthuthukiswa kwemboni yezinkukhu. Lesi sifo sinomlando omude futhi sihlala sibhekene nezigameko ezintsha, njengeqembu elincane le-Avian leukemia virus J (ALV-J), elatholwa futhi lahlonzwa ngasekupheleni kwawo-1980 e-UK njengohlobo olusha lwegciwane le-avian leukemia, elidala umonakalo omkhulu embonini yezinkukhu.
Isimiso Sokuhlolwa
Ikhithi isebenzisa indlela yesangweji i-ELISA, i-anti-avian leukocyte P27 i-monoclonal antibody ehlanzekile ifakwe ngaphambili emicupheni ye-enzyme micro-well. Ekuhlolweni, i-antigen esesampula iboshelwe ku-antibody epuleti elihlanganisiwe, ngemva kokugeza ukuze kukhishwe i-antigen engaboshiwe nezinye izakhi, i-enzyme ye-monoclonal antibody yengezwe ku-antigen-antibody yokuhlola ngokukhethekile. bese kuyawashwa, i-enzyme engaboshiwe i-conjugate iyasuswa, isixazululo se-TMB substrate sengezwa ku-microplate, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis iyingxenye eqondile yokuqukethwe kwamasosha omzimba kusampula. Engeza isixazululo sokumisa, Ngemva kokusabela, ukumuncwa kwenani elingu-A emthonjeni wokusabela kukalwa nge-wavelength engu-450 nm.
Okuqukethwe
| I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
| 1 |
| 1e/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2 eya/4 | |
| 10 | i-serum dilution microplate | 1e/2e | |
| 11 | Isiyalezo | 1 ama-pcs |
