Faka i-reagent ku-100ml wamanzi isampula, ngemva kokuncibilika, fukamela ku-36°C amahora angu-24.
Ukuhunyushwa kwemiphumela:
engenambala = negative
Okuphuzi = okuhle kuma-coliforms aphelele
Ophuzi + fluorescence = Escherichia coli positive.
Engeza ama-reagents kusampula yamanzi futhi uhlanganise kahle.
Thela epuletini lokuthola inani lemithombo engu-51 (umthamo wepuleti lomthombo) noma ipuleti lokuthola umthamo wemithombo engu-97 (ipuleti lomthombo lobuningi)
Sebenzisa umshini wokuvala olinganiselayo olawulwa uhlelo
ukuvala i-quantitative discovery disc (umthamo wepuleti lomthombo) ukuze uvalwe futhi ufukamele ku-36°C amahora angu-24.
Isiko le-coliform elikwazi ukumelana nokushisa/i-fecal coliform elingu-44.5°C amahora angu-24 liphuzi futhi lilungile.
Ukuhunyushwa kwemiphumela:
engenambala = negative
I-checkered ephuzi = ama-coliform aphelele
Igridi ephuzi + ye-fluorescent = I-Escherichia coli ireferensi enhle yokubala kwetafula le-MPN