Imikhiqizo
Ikhithi yokuhlola ye-Lifecosm Feline Parvovirus Ag
Ikhithi yokuhlola ye-Feline Parvovirus Ag
| Inombolo yekhathalogi | I-RC-CF16 |
| Isifinyezo | Ukutholwa kwama-antigen athile e-FPV phakathi nemizuzu eyi-10 |
| Isimiso | Isinyathelo esisodwa sokuhlolwa kwe-immunochromatographic |
| Okuhloswe ngakho Ukuthola | Ama-antigen e-FPV |
| Isampula | Indle Yezilwane |
| Isikhathi sokufunda | 5 ~ 10 imizuzu |
| Ukuzwela | I-FPV: 100.0 % vs. PCR, |
| Ukucaciswa | I-FPV : 100.0 % vs. PCR |
| Okuqukethwe | Ikhithi yokuhlola, Amashubhu, izinto ezilahlayo, kanye nokotiniama-swabs |
| Isitoreji | Ithempelesha Yegumbi (ku-2 ~ 30℃) |
| Ukuphelelwa yisikhathi | Izinyanga ezingama-24 ngemuva kokukhiqiza |
| Isexwayiso | Sebenzisa phakathi kwemizuzu eyi-10 ngemuva kokuvulaSebenzisa inani elifanele lesampula (0.1 ml ye-dropper) Sebenzisa ngemva kwemizuzu engu-15–30 e-RT uma egcinwe ngaphansi kwezimo ezibandayo Cabangela imiphumela yokuhlolwa njengengavumelekile ngemva kwemizuzu engu-10 |
Ulwazi
I-Feline parvovirus igciwane elingabangela izifo ezinzima emakatini - ikakhulukazi amakati.Kungaba yingozi.Kanye ne-feline parvovirus (FPV), lesi sifo saziwa nangokuthi i-feline infectious enteritis (FIE) kanye ne-feline panleukopenia.Lesi sifo senzeka emhlabeni wonke, futhi cishe wonke amakati avezwa unyaka wawo wokuqala ngoba igciwane lizinzile futhi litholakala yonke indawo.
Amakati amaningi athola i-FPV endaweni engcolile esebenzisa indle ethelelekile kunamakati anamagciwane.Igciwane lingase futhi ngezinye izikhathi lisabalale ngokuthinta izingubo zokulala, izitsha zokudla, noma ngisho nabaphathi bamakati anesifo.
Futhi, Ngaphandle kokwelashwa, lesi sifo ngokuvamile siyabulala.
I-Parvovirus.Electron Micrograph from Stewart McNulty, Queens University, Belfast.
Izimpawu
Izimpawu zokuqala umnikazi angase aziqaphele ukucindezeleka okuvamile, ukulahlekelwa ukudla, ukushisa okukhulu, ukukhathala, ukuhlanza, ukuphelelwa amanzi emzimbeni, kanye nokulenga phezu kwesitsha samanzi.Inkambo yesifo ingase ibe mfushane futhi iqhume.Izimo ezithuthukile, lapho zitholwa, zingase zibangele ukufa phakathi namahora.Ngokuvamile, ukugula kungase kuqhubeke izinsuku ezintathu noma ezine ngemva kokuphakama kokuqala kwezinga lokushisa lomzimba.
Umkhuhlane uzoshintshashintsha phakathi nokugula futhi uvele wehlele emazingeni angejwayelekile ngaphambi nje kokufa.Ezinye izimpawu ezigabeni zakamuva kungaba isifo sohudo, i-anemia, nokuhlanza okungapheli.
I-FPV idlange kakhulu futhi izimpawu ziyahlukahluka kangangokuthi noma yiliphi ikati eligulayo kufanele liyiswe kudokotela wezilwane ukuze lixilongwe.
Ukuxilongwa nokwelashwa
Empeleni, ukutholwa kwe-antigen ye-FPV endle kuvame ukwenziwa kusetshenziswa i-latex agglutination etholakalayo kwezohwebo noma ukuhlolwa kwe-immunochromatographic.Lezi zivivinyo zinokuzwela nokucacisa okwamukelekile uma kuqhathaniswa nezindlela zokubhekisela.
Ukuxilongwa nge-electron microscopy kulahlekelwe ukubaluleka kwakho ngenxa yezinye izindlela ezisheshayo nezizenzekelayo.Amalabhorethri akhethekile ahlinzeka ngokuhlolwa okusekelwe ku-PCR egazini eliphelele noma indle.Igazi eliphelele liyanconywa kumakati angenaso isifo sohudo noma lapho kungekho amasampula endle atholakalayo.
Ama-antibodies ku-FPV angaphinde atholwe yi-ELISA noma i-Immunofluorescence engaqondile.Kodwa-ke, ukusetshenziswa kokuhlolwa kwe-antibody kunenani elilinganiselwe, ngoba ukuhlolwa kwe-serological akuhlukanisi phakathi kwamasosha omzimba abangelwa ukutheleleka kanye nokugoma.
Alikho ikhambi le-FPV kodwa uma lesi sifo sitholakala ngesikhathi, izimpawu zingelapheka futhi amakati amaningi alulama ngokunakekelwa okujulile okuhlanganisa ukuncelisa okuhle, ukwelapha uketshezi kanye nokuncelisa.Ukwelapha kuhilela ukunciphisa ukuhlanza nesifo sohudo, ukuvimbela ukuphelelwa amanzi emzimbeni okulandelayo, kanye nezinyathelo zokuvimbela ukutheleleka kwesibili kwamagciwane, kuze kube yilapho isimiso somzimba sokuzivikela ezifweni sekati sithatha izintambo.
Ukuvimbela
Ukugoma kuyindlela eyinhloko yokuvimbela.Izifundo zokugoma eziyisisekelo ngokuvamile ziqala emasontweni ayisishiyagalolunye ubudala ngomjovo wesibili lapho emasontweni ayishumi nambili ubudala.Amakati amadala kufanele athole ama-booster onyaka.Umuthi wokugomela i-FPV awunconyelwe amakati angaphansi kwamasonto ayisishiyagalombili ubudala, njengoba ukungatheleleki kwawo kwemvelo kungase kuphazamise ukusebenza komgomo we-FPV.
Njengoba igciwane le-FPV liqine kakhulu, futhi lingaqhubeka lihlala emvelweni izinyanga noma iminyaka, kufanele kubulawe amagciwane kuyo yonke indawo ngemva kokugqashuka kwe-feline panleukopenia endlini ehlanganyelwa amakati.
Ukuxilongwa
Ukuhlola kokuqala okuthandwayo ukuhlolwa kwe-soluble-antigen, okufana ne-ELISA nokunye ukuhlola kwe-immunochromatographic, okuthola i-antigen yamahhala oketshezini.Ukuhlolwa kwesifo kungenziwa kalula.Ukuhlolwa kwe-Soluble-antigen kuthembeke kakhulu lapho kuhlolwa i-serum noma i-plasma, esikhundleni segazi eliphelele.Ezilungiselelweni zokuhlola amakati amaningi azoba nemiphumela emihle ngokuhlolwa kwe-soluble-antigen ngaphakathi
izinsuku ezingama-28 ngemuva kokuchayeka;kodwa isikhathi phakathi kokuchayeka kanye nokuthuthukiswa kwe-antigenemia sishintsha kakhulu futhi singase sibe side kakhulu kwezinye izimo.Ukuhlola okusebenzisa amathe noma izinyembezi kuveza iphesenti eliphezulu ngokungamukeleki lemiphumela engalungile futhi ukusetshenziswa kwakho akunconywa.Uma kutholakala ukuthi i-feline ayinaso lesi sifo umgomo wokuvimbela unganikezwa.Umgomo, ophindwa kanye ngonyaka, unezinga lempumelelo eliphezulu ngendlela emangalisayo futhi njengamanje (uma lingekho ikhambi elisebenzayo) uyisikhali esinamandla kakhulu ekulweni ne-feline leukemia.
Ukuvimbela
Okuwukuphela kwendlela eqinisekile yokuvikela amakati ukuvikela ukuchayeka kwawo egciwaneni.Ukulunywa kwekati kuyindlela enkulu yokutheleleka ngegciwane, ngakho ukugcina amakati esendlini- futhi kude namakati angase abe negciwane angase awalume - kunciphisa ngokuphawulekayo amathuba awo okuthola i-FIV.Ukuze kuphephe amakati ahlala kuwo, amakati angenalo ukutheleleka kuphela okufanele angeniswe ekhaya elinamakati angenalo igciwane.
Imithi yokugoma esiza ukuvikela ekuthelelekeni nge-FIV isiyatholakala.Kodwa-ke, akuwona wonke amakati agonyiwe azovikelwa umgomo, ngakho ukuvimbela ukuchayeka kuzohlala kubalulekile, ngisho nasezifuyweni ezigonyiwe.Ngaphezu kwalokho, ukugoma kungase kube nomthelela emiphumeleni yokuhlolwa ye-FIV yesikhathi esizayo.Kubalulekile ukuthi uxoxe ngezinzuzo kanye nokubi kokugoma nodokotela wakho wezilwane ukuze akusize unqume ukuthi ingabe imigomo ye-FIV kufanele inikezwe ikati lakho.
