Inombolo yekhathalogi | I-RC-CF29 |
Isifinyezo | Ukutholwa kwama-Canine Dirofilaria immitis antigens, amasosha omzimba e-Anaplasma, amasosha omzimba E. canis phakathi nemizuzu eyi-10 |
Isimiso | Isinyathelo esisodwa sokuhlolwa kwe-immunochromatographic |
Okuhloswe ngakho Ukuthola | CHW Ag : Dirofilaria immititis antigens Anapalsma Ab : Amasosha omzimba e-AnaplasmaE. canis Ab : Amasosha omzimba E. canis |
Isampula | I-Canine Igazi Eliphelele, I-Plasma noma I-Serum |
Isikhathi sokufunda | Imizuzu eyi-10 |
Ubuningi | Ibhokisi elingu-1 (ikhithi) = amadivaysi ayi-10 (Ukupakishwa komuntu ngamunye) |
Okuqukethwe | Ikhithi yokuhlola, ibhodlela le-Buffer, kanye ne-dropper elahlayo |
Isitoreji | Ithempelesha Yegumbi (ku-2 ~ 30℃) |
Ukuphelelwa yisikhathi | Izinyanga ezingama-24 ngemuva kokukhiqiza |
Isexwayiso | Sebenzisa phakathi kwemizuzu eyi-10 ngemuva kokuvulaSebenzisa inani elifanele lesampula (0.01 ml ye-dropper) Sebenzisa ngemva kwemizuzu engu-15–30 e-RT uma egcinwe ngaphansi kwezimo ezibandayo Cabangela imiphumela yokuhlolwa njengengavumelekile ngemva kwemizuzu engu-10 |
Izikelemu zenhliziyo zabantu abadala zikhula ngamasentimitha ambalwa ubude futhi zihlala emithanjeni yamaphaphu lapho zingathola khona imisoco eyanele.Izikelemu zenhliziyo ngaphakathi emithanjeni zibangela ukuvuvukala futhi zenze i-hematoma.Inhliziyo-ke kufanele iphampe kaningi kunangaphambili njengoba izikelemu zenhliziyo zanda ngenani, zivale imithambo.
Uma ukutheleleka kukhula (ngaphezu kwama-heartworms angu-25 enja engu-18 kg), izikelemu zenhliziyo zingena ku-atrium engakwesokudla, zivimbe ukugeleza kwegazi.
Lapho inani lama-heartworms lifinyelela ngaphezu kwama-50, bangahlala
ama-atrium nama-ventricles.
Lapho itheleleka ngezikelemu zenhliziyo ezingaphezu kwe-100 engxenyeni efanele yenhliziyo, inja ilahlekelwa ukusebenza kwenhliziyo futhi ekugcineni iyafa.Kuyabulala lokhu
I-phenomenon ibizwa ngokuthi "i-Caval Syndrom."
Ngokungafani nezinye izilokazane, izikelemu zenhliziyo zibeka izinambuzane ezincane ezibizwa ngokuthi i-microfilaria.I-Microfilaria kumiyane ingena enjeni lapho umiyane umunca igazi enjeni.Ama-heartworms angaphila kumphathi iminyaka engu-2 ayafa uma engathutheli komunye umsingathi phakathi naleso sikhathi.Amagciwane ahlala enjeni ekhulelwe angakwazi ukuthelela umbungu wayo.
Ukuhlolwa kusenesikhathi kwezikelemu zenhliziyo kubaluleke kakhulu ekuziqedeni.Izikelemu zenhliziyo zidlula ezinyathelweni ezimbalwa ezifana ne-L1, L2, L3 kuhlanganise nesiteji sokudlulisela ngomiyane ukuze zibe izikelemu zenhliziyo zabantu abadala.
I-Microfilaria kumiyane ikhula ibe izimuncagazi ze-L2 kanye ne-L3 ezikwazi ukuthelela izinja emasontweni ambalwa.Ukukhula kuncike esimweni sezulu.Izinga lokushisa elilungile le-parasite lingaphezu kuka-13.9 ℃.
Lapho umiyane onaleli gciwane luluma inja, i-microfilaria ye-L3 ingena esikhunjeni sayo.Esikhumbeni, i-microfilaria ikhula ibe yi-L4 amaviki angu-1 ~ 2.Ngemva kokuhlala esikhumbeni izinyanga ezi-3, i-L4 ikhula ibe yi-L5, engena egazini.
I-L5 njengohlobo lwe-heartworm yabantu abadala ingena enhliziyweni nasemithanjeni yamaphaphu lapho ezinyangeni ezingu-5 ~ 7 kamuva izikelemu zenhliziyo zibeka izinambuzane.
Ukutheleleka kwe-heartworms kwelapheka ngempumelelo ezimweni eziningi.Ukuqeda zonke izikelemu zenhliziyo, ukusetshenziswa kwemithi kuyindlela engcono kakhulu.Ukutholwa kusenesikhathi kwezikelemu zenhliziyo kuphakamisa izinga lempumelelo yokwelashwa.Kodwa-ke, esigabeni sokugcina sokutheleleka, kungenzeka kube nezinkinga, okwenza ukwelashwa kube nzima kakhulu.
Ibhaktheriya i-Anaplasma phagocytophilum (ngaphambilini eyayaziwa ngokuthi i-Ehrilichia phagocytophila) ingase ibangele ukutheleleka ezinhlobonhlobo zezilwane eziningana kuhlanganise nabantu.Lesi sifo esitholakala ezilwaneni ezifuywayo sibizwa nangokuthi i-tick-borne fever (TBF), futhi saziwa okungenani iminyaka engu-200.Amagciwane omndeni wakwa-Anaplasmataceae ane-gram-negative, nonmotile, coccoid to ellipsoid organisms, ahluka ngosayizi ukusuka ku-0.2 kuya ku-2.0um ububanzi.Ziyi-aerobes ephoqelekile, ayinayo indlela ye-glycolytic, futhi zonke ziyizilokazane ze-intracellular.Zonke izinhlobo zezinhlobo ze-Anaplasma zihlala kuma-vacuoles ane-membrane-lined kumaseli angakakhuli noma avuthiwe e-hematopoietic host host mammalian.I-phagocytophilum ihlasela ama-neutrophils kanti igama elithi granulocytotropic libhekisela kuma-neutrophils angenwe yileli gciwane.Izinto eziphilayo ezingavamile, ziye zatholakala kuma-eosinophils.
I-Anaplasma phagocytophilum
Izimpawu zomtholampilo ezivamile ze-canine anaplasmosis zihlanganisa ukushisa okukhulu, ukukhathala, ukucindezeleka kanye ne-polyarthritis.Izimpawu ze-neurologic (ataxia, seizures kanye nobuhlungu bentamo) nazo zingabonakala.Ukutheleleka kwe-Anaplasma phagocytophilum akuvamile ukubulawa ngaphandle uma kuyinkimbinkimbi ngezinye izifo.Ukulahlekelwa okuqondile, izimo ezikhubazekile kanye nokulahlekelwa kokukhiqiza kuye kwabonwa emazinyaneni.Ukukhipha isisu kanye ne-spermatogenesis ekhubazekile ezimvini nasezinkomeni kubhaliwe.Ubukhulu bokutheleleka buthonywa izici ezimbalwa, njengokuhlukahluka kwe-Anaplasma phagocytophilum ehilelekile, amanye amagciwane, ubudala, isimo sokuzivikela komzimba nesimo somsingathi, nezici ezifana nesimo sezulu nokuphathwa.Kufanele kukhulunywe ukuthi ukubonakaliswa komtholampilo kubantu kusuka ekuguleni okuncane okulinganiselwe okufana nomkhuhlane, kuya ekuthelelekeni okusongela ukuphila.Kodwa-ke, izifo eziningi zabantu cishe ziholela ekubonakalisweni komtholampilo okuncane noma kungabikho nhlobo.
I-Anaplasma phagocytophilum idluliselwa ngemikhaza ye-ixodid.E-United States ama-vector ayinhloko i-Ixodes scapularis ne-Ixodes pacificus, kuyilapho i-Ixode ricinus itholakale iyi-exophilic vector eyinhloko eYurophu.I-Anaplasma phagocytophilum isakazwa ngokudlulela yile mikhaza ye-vector, futhi abukho ubufakazi bokudluliselwa kwe-transovarial.Ucwaningo oluningi kuze kube manje oluphenye ngokubaluleka kwezilwane ezincelisayo ze-A. phagocytophilum kanye nezindawo ezithwala imikhaza zigxile kumagundane kodwa lesi silwane sinohlu olubanzi lwezilwane ezincelisayo, ezithelela amakati afuywayo, izinja, izimvu, izinkomo, namahhashi.
I-Indirect immunofluorescence assay ukuhlolwa okuyinhloko okusetshenziselwa ukuthola ukutheleleka.Amasampula e-serum yesigaba se-acute ne-convalescent angahlolwa ukuze kubhekwe ushintsho oluphindwe kane ku-antibody titer kuya ku-Anaplasma phagocytophilum.I-intracellular inclusions (morulea) ibonakala kuma-granulocyte ku-Wright noma ku-Gimsa enegazi elingcolisiwe.Izindlela ze-Polymerase chain reaction(PCR) zisetshenziselwa ukuthola i-Anaplasma phagocytophilum DNA.
Awukho umgomo otholakalayo wokuvikela ukutheleleka kwe-Anaplasma phagocytophilum.Ukuvimbela kuncike ekuchayekeni kwe-ejenti yomkhaza (i-Ixodes scapularis, i-Ixodes pacificus, ne-Ixode ricinus) kusukela entwasahlobo kuya ekwindla, ukusetshenziswa kokuvimbela ama-anticaricides, kanye nokusetshenziswa kwe-prophylactic ye-doxycycline noma i-tetracycline uma uvakashela i-Ixodes scapularis, i-Ixodes pacide-ricinus, kanye ne-tick izifunda ezingapheli.
I-Ehrlichia canis izinambuzane ezincane ezimise okwenduku ezisakazwa umkhaza wenja ensundu, i-Rhipcephalus sanguineus.I-E. canis iyimbangela ye-ehrlichiosis yakudala ezinjeni.Izinja zingangenwa yi-Ehrlichia spp eminingana.kodwa evame kakhulu ebangela i-canine ehrlichiosis i-E. canis.
I-E. canis manje yaziwa ukuthi isisakazekele kulo lonke elase-United States, eYurophu, eNingizimu Melika, e-Asia naseMedithera.
Izinja ezithelelekile ezingelashwa zingaba abathwali besifo iminyaka eminingi futhi ekugcineni zife ngenxa yokopha okukhulu.
Ukutheleleka kwe-Ehrlichia canis ezinjeni kuhlukaniswe izigaba ezi-3;
ISIGABA ESIYI-ACUTE: Lesi ngokuvamile isigaba esithambile kakhulu.Inja ngeke isakwazi ukudla, ingadli, futhi kungenzeka ibe nama-lymph nodes akhulisiwe.Kungase kube nomkhuhlane futhi kodwa akuvamile ukuthi lesi sigaba sibulale inja.Iningi lizihlanza ngokwazo kodwa abanye bazodlulela esigabeni esilandelayo.
ISIGABA ESIPHANSI: Kulesi sigaba, inja ibonakala ijwayelekile.I-organism i-sequestered ku-spleen futhi empeleni ifihlekile lapho.
ISIGABA ESINGAPHELI: Kulesi sigaba inja iyagula futhi.Kufika ku-60% wezinja ezingenwe yi-E. canis zizopha ngendlela engavamile ngenxa yokuncipha kwezinombolo zamaplatelet.Ukuvuvukala okujulile emehlweni okubizwa ngokuthi "uveitis" kungase kwenzeke ngenxa yokuvuselela umzimba isikhathi eside.Imiphumela ye-Neurologic ingase ibonakale.
Ukuxilongwa okuqondile kwe-Ehrlichia canis kudinga ukubonakala ngeso lengqondo kwe-morula ngaphakathi kwe-monocyte ku-cytology, ukutholwa kwamasosha omzimba e-E. canis serum ngokuhlolwa okungaqondile kwe-immunofluorescence antibody test (IFA), i-polymerase chain reaction (PCR) amplification, kanye/noma i-gel blotting (i-Western immunoblotting).
Umgomo oyinhloko wokuvimbela i-canine ehrlichiosis ukulawula imikhaza.Umuthi okhethwayo wokwelapha zonke izinhlobo ze-ehrlichiosis yi-doxycycline okungenani inyanga eyodwa.Kufanele kube nokuthuthuka okumangalisayo komtholampilo kungakapheli amahora angama-24-48 kulandela ukuqaliswa kokwelashwa ezinjeni ezinesigaba esibucayi noma esibuthakathaka sesigaba esingamahlalakhona.Ngalesi sikhathi, izibalo zamaplatelet ziqala ukwanda futhi kufanele zibe ngokwejwayelekile phakathi nezinsuku eziyi-14 ngemuva kokuqala kokwelashwa.
Ngemuva kokutheleleka, kungenzeka ukuthi uphinde utheleleke;ukuzivikela komzimba akuhlali ngemva kokutheleleka kwangaphambilini.
Ukuvimbela okungcono kakhulu kwe-ehrlichiosis ukugcina izinja zingenayo imikhaza.Lokhu kufanele kufake phakathi ukuhlola isikhumba nsuku zonke ukuze uthole imikhaza nokwelapha izinja ngokulawula imikhaza.Njengoba imikhaza ithwala ezinye izifo ezibhubhisayo, njengesifo i-Lyme, i-anaplasmosis kanye ne-Rocky Mountain spotted fever, kubalulekile ukugcina izinja zingenayo imikhaza.