Imikhiqizo
I-Lifecosm Canine Brucellosis Ag Ikhithi Yokuhlola Esheshayo
Ikhithi Yokuhlola ye-LSH Ab
| I-Brucella Ag Test Kit | |
| Inombolo yekhathalogi | I-RC-CF10 |
| Isifinyezo | Ukutholwa kwe-antigen ethile ye-Brucella phakathi nemizuzu eyi-10 |
| Isimiso | Isinyathelo esisodwa sokuhlolwa kwe-immunochromatographic |
| Okuhloswe ngakho Ukuthola | I-Brucella antigen |
| Isampula | I-Canine, i-bovine ne-Ovis Igazi Eliphelele, i-Plasma noma iSerum |
| Isikhathi sokufunda | 10 ~ 15 imizuzu |
| Ukuzwela | 91.3 % vs. IFA |
| Ukucaciswa | 100.0 % vs. IFA |
| Umkhawulo Wokutholwa | I-IFA Titer 1/16 |
| Ubuningi | Ibhokisi elingu-1 (ikhithi) = amadivaysi ayi-10 (Ukupakishwa komuntu ngamunye) |
| Okuqukethwe | Ikhithi yokuhlola, Amashubhu, izinto ezilahlayo |
|
Isexwayiso | Sebenzisa phakathi kwemizuzu eyi-10 ngemuva kokuvula Sebenzisa inani elifanele lesampula (0.01 ml ye-dropper) Sebenzisa ngemva kwemizuzu engu-15–30 e-RT uma egcinwe ngaphansi kwezimo ezibandayo Cabangela imiphumela yokuhlolwa njengengavumelekile ngemva kwemizuzu engu-10 |
Ulwazi
I-Brucella iyilungu lomndeni i-Brucellaceae futhi ihlanganisa izinhlobo eziyishumi ezincane, ezingenayo i-motile, ezingenayo i-sporing, i-aerobic, i-gram-negative intracellular coccobacilli.Ziyi-catalase, i-oxidase kanye ne-urea positive bacteria.Amalungu ohlobo angakhula emithonjeni yezindaba ethuthukisiwe njenge-agar yegazi noma i-chocolate agar.I-Brucellosis i-zoonosis eyaziwa kakhulu, ekhona kuwo wonke amazwekazi, kodwa ehlukahlukene kakhulu kanye nezigameko, ezilwaneni nakubantu.I-Brucella, njengama-facultative intracellular parasites, ihlanganisa izinhlobo eziningi zezilwane ezihlala emphakathini ngendlela engapheli, ngokunokwenzeka ehlala njalo, mhlawumbe impilo yazo yonke.
I-Brucella colony ukubukeka
Ukudluliswa
Izinhlobo ze-Brucella zivame ukudluliselwa phakathi kwezilwane ngokuthintana ne-placenta, i-fetus, uketshezi lwe-fetus kanye nokuphuma kwesitho sangasese sowesifazane.isilwane esinegciwane.Iningi noma zonke izinhlobo zeBrucella nazo zitholakala esidodeni.Iduna lingachitha lezi zinambuzane isikhathi eside noma impilo yonke.Ezinye izinhlobo ze-Brucella nazo zitholakale kwezinye izimfihlo kanye nokuphumayo okuhlanganisa umchamo, indle, i-hygroma fluid, i-salvia, ubisi kanye nokukhishwa kwekhala kanye ne-ocular.
I-Ecology yokutheleleka kwe-zoonotic Brucella
Izimpawu
Ezinkomeni
Ayikho indlela ephumelelayo yokubona izilwane ezinamagciwane ngokubukeka kwazo.Izimpawu ezisobala kakhulu ezilwaneni ezikhulelwe ukukhipha isisu noma ukuzalwa kwamathole abuthakathaka.Ukukhiqizwa kobisi kungase kuncishiswe kusukela ekushintsheni esikhathini esivamile sokuncelisa okubangelwa ukukhipha izisu kanye nokumitha okulibazisekayo.Ezinye izimpawu ze-brucellosis zihlanganisa ukwehla okubonakalayo kokuzala okunezinga eliphansi lokukhulelwa, ukugcinwa kokuzalwa ngemva kokuzalwa okubangelwa izifo zesibeletho, kanye (ngezikhathi ezithile) nokunwetshwa, amalunga e-arthritis.
Ezinjeni
Ezinjeni, amagciwane e-Brucellosis ajwayele ukuhlala esithweni sangasese kanye nesistimu ye-lymphatic, kodwa kungenzeka ukuthi asakaze ezinso, amehlo kanye ne-intervertebral disc.Lapho i-brucellosis ihlasela i-intervertebral disc, umphumela uba i-discopondylitis.Ezinjeni, izimpawu ezivela ezithweni zokuzala zivamile.Izinja zesilisa, ngokwesibonelo, zingaba nokuvuvukala kwe-scrotal kanye ne-testicular, kuyilapho izinja zabesifazane zingaphuphunyelwa yizisu.Imfiva ayivamile, kodwa ubuhlungu obuhlobene ne-Brucellosis bungenza inja ibe buthaka.Uma lesi sifo sisakazeka ezinso, amehlo noma izimpawu ze-intervertebral disc zingaqala ukukhombisa kulezi zitho.
Ezingulubeni
Isikhathi esiphakathi kokutheleleka kanye nokuvela kwezimpawu zomtholampilo zesifo singasukela cishe isonto eli-1 kuye ezinyangeni ezi-2.Izimpawu zokuthi umhlambi usunegciwane ikakhulukazi lezo zokwehluleka ukuzala - ukukhipha izisu, ukubuyela emsebenzini ngemva kokukhwelana kanye nokuzalwa kwezingulube ezibuthakathaka noma ezizalwa sezifile.Ezinye izingulube zingase zibe nokutheleleka esibelethweni futhi zibonise ukugobhoza esithweni sangasese.Izingulube ezinamagciwane zingase zibe namasende avuvukele.Bobabili ubulili bungase buqhuge bube namalunga avuvukele kanye/noma bube nezimpawu zokungaxhumani kahle nokukhubazeka kwemilenze yangemuva.
Ukuxilongwa
1.Ukuhlukaniswa nokuhlonza i-ejenti
Izinhlobo ze-Brucella zingatholakala ezicutshini eziningi kanye nokuphuma koketshezi oluningi, ikakhulukazi ulwelwesi lwengane, uketshezi lwesitho sangasese sowesifazane, ubisi (noma ukukhishwa kombele), isidoda, isifo samathambo soketshezi lwe-hygroma, kanye nokuqukethwe kwesisu, ubende namaphaphu okuvela ku-fetus ehushuliwe.Iningi lezinhlobo ze-Brucella ezivela emakoloni phakathi nezinsuku ezimbalwa kumidiya ekhethiwe.Lapho amapuleti ebhekwa emini ngokusebenzisa into esobala, la makoloni ayashintshashintsha futhi anombala woju oluphaphathekile.Uma ubhekwa phezulu, amakoloni abonakala e-convex futhi emhlophe njengeparele.Kamuva amakoloni aba makhudlwana futhi abe mnyama kancane.
2.Nucleic acid Indlela
I-PCR iyithuluzi elikahle lokuxilongwa kwe-brucellosis.Izivivinyo eziningi ezisekelwe ku-PCR zenzelwe ukuhlonza i-Brucella ukuze kuthuthukiswe amandla okuxilonga.Ukuhlolwa kwe-PCR okukhethekile kohlobo lwanele ukuhlonza i-Brucella.
3.Ukuxilongwa kwe-serological
Ziningi izivivinyo ze-serological.Ukuhlolwa kwe-serological okuvamise ukusetshenziselwa ukuhlola inkomo ngayinye noma umhlambi kuhlanganisa ukuhlolwa kwe-Brucella antigen, ukulungiswa komphelelisi, ama-enzyme-linked immunosorbent assays(ELISA) angaqondile noma ancintisanayo.
