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Inkukhu Infectious Bursal Disease Virus Ab Elisa kit Isithombe Esifakiwe
  • I-Chicken Infectious Bursal Disease Virus Ab Elisa kit
  • I-Chicken Infectious Bursal Disease Virus Ab Elisa kit

I-Chicken Infectious Bursal Disease Virus Ab Elisa kit

Ikhodi Yomkhiqizo:

Igama Lento: Inkukhu Ethathelwanayo I-Bursal Disease Virus Ab Elisa kit

Isifinyezo: Ikhithi yokuhlonza igciwane le-bursal virus yenkukhu ethathelwanayo ukuze kutholwe igciwane le-bursal disease elithathelwanayo linciphisa amasosha omzimba ku-serum yenkukhu ukuze kuhlolwe amasosha omzimba akhiqizwa ukugonywa kokugoma kwesifo se-bursal esithathelwanayo Isimo kanye nokuxilonga izinkukhu ezinegciwane.

Okuhlosiwe: I-Chicken infectious bursal virus antibody

Isampula Lokuhlola: I-Serum

Ukucaciswa: 1 kit = 192 Test

Isitoreji: Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi.

Isikhathi seshelufu: izinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.


IKHATHALOGU

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

I-Chicken Infectious Bursal Disease Virus Ab Elisa kit

Isifinyezo  Ukutholwa kwe-antibody eyenza i-neutralize ngokumelene ne-bursa ethelelanayo ye-Fabricius virus ku-serum yenkukhu
Okuhloswe ngakho Ukuthola I-anti-virus ye-bursal virus yenkukhu
Isampula I-Serum

 
Ubuningi 1 kit = 192 Test
 

 

Ukuzinza kanye Nesitoreji

1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi.

2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.

 

 

 

Ulwazi

Isifo se-bursal esithathelwanayo(IBD), eyaziwa nangokuthi isifo seGumboro, i-infectious bursitis kanye ne-infectious avian nephrosis, isifo esithathelwanayo kakhulu entsheni.izinkukhukanye nama-turkeys abangelwa igciwane le-infectious bursal disease (IBDV), elibonakala ngokuthiimmunosuppressionkanye nokufa ngokuvamile emavikini angu-3 kuya kwangu-6 ubudala.Lesi sifo satholwa okokuqala ngoGumboro, DelawareNgo-1962. Kubaluleke kakhulu kwezomnotho embonini yezinkukhu emhlabeni wonke ngenxa yokwanda kokungenwa ezinye izifo kanye nokuphazamiseka okungekuhle kokusebenza ngempumelelo.ukugoma.Eminyakeni yamuva nje, kuye kwavela izinhlobo ezinonya kakhulu ze-IBDV (vvIBDV), ezibangela ukufa okukhulu kwenkukhu, eYurophu,i-Latin America,I-South-East Asia, Afrika kanyeEmaphakathi Mpumalanga.Ukutheleleka kungena emzileni we-oro-fecal, lapho inyoni ethintekile ikhipha amazinga aphezulu egciwane cishe amasonto amabili ngemuva kokutheleleka.Lesi sifo sisakazeka kalula sisuka ezinkukhwini ezinegciwane siye ezinkukhwini ezinempilo ngokudla, amanzi, nangokuthintana ngokomzimba.

Isimiso Sokuhlolwa

Ikhithi isebenzisa indlela ye-ELISA yokuncintisana, iphrotheni ye-VP2 yegciwane le-bursal efakwe ngaphambilini ku-microplate, futhi iqhudelane ne-anti-VP2 protein antibody ku-serum ye-vector yesigaba esiqinile kusetshenziswa i-anti-VP2 protein monoclonal antibody.Ekuhlolweni, i-monoclonal antibody ezohlolwa kanye ne-anti-VP2 protein yengezwe, futhi ngemva kokufukamela, uma isampula iqukethe igciwane le-bursal disease elithathelwanayo lenkukhu i-VP2 protein-specific antibody, libophezela ku-antigen epuleti elimboziwe.Ngaleyo ndlela kuvimbela ukuboshwa kwe-anti-VP2 protein monoclonal antibody ku-antigen, ngemva kokugeza ukuze kukhishwe i-antibody engaboshiwe nezinye izakhi;bese wengeza i-enzyme emele igundane enelebula i-antibody yesibili ukuze ibophezele ngokukhethekile ku-antigen-antibody complex epuleti lokubona;I-conjugate ye-enzyme engaboshiwe isuswa ngokugeza;i-substrate ye-TMB yengezwa ku-microwell ukuze kuthuthukiswe umbala, futhi inani lokumunca lesampula lihlotshaniswa kabi nokuqukethwe kwe-anti-VP2 protein antibody equkethwe kuyo, ngaleyo ndlela kufezwe injongo yokuthola i-anti-VP2 protein antibody kusampula.

Okuqukethwe

 

I-Reagent

Ivolumu

96 Izivivinyo/192Izivivinyo
1
I-microplate ehlanganiswe ne-antigen

 

1e/2e
2
 Ukulawula Okungalungile

 

2.0ml
3
 Ukulawula Okuhle

 

1.6ml
4
 Amasampula e-diluent

 

100ml
5
Isixazululo sokugeza (kugxilwe ku-10X)

 

100ml
6
 Ukuhlanganiswa kwe-enzyme

 

11/22ml
7
 I-substrate

 

11/22ml
8
 Isixazululo sokumisa

 

15ml
9
I-Adhesive plate sealer

 

2 eya/4
10 i-serum dilution microplate

1e/2e
11  Isiyalezo

1 ama-pcs

 

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