Isifinyezo | Ukutholwa kwe-Antibody ethize Yezinyawo Nezifo Zomlomo I-BRU |
Isimiso | Ikhithi yokuhlola ye-BRU ye-ELISA yokuthola amasosha omzimba e-Brucellosis ku-serum yezingulube, izinkomo, izimvu nezimbuzi. |
Okuhloswe ngakho Ukuthola | I-antibody ye-BRU |
Isampula | I-Serum
|
Ubuningi | 1 kit = 192 Test |
Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
|
I-Brucellosis isifo esithathelwanayo kakhului-zoonosisokubangelwa ukungenwa yi-unpasteurizedubisinoma kungaphekwa kahleinyamaezilwaneni ezithelelekile, noma ukuthintana eduze nokuphuma kwazo.
Yaziwa nangokuthi i-undulant fever, i-Malta fever, kanye ne-Mediterranean fever.
I-BRU amasosha omzimbaELISAikhithi yokuhlolajwayele ukutholakala kwama-antibodies e-Brucellosis ku-serum yezingulube, izinkomo,sheep kanye nembuzi .
Sebenzisa le kit indlela ye-ELISA yokuncintisana ngaphambi kokugqokai-BRU ama-antigens emithonjeni ye-microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululiwe futhii-enzyme ebizwa ngokuthi i-anti-I-BRU I-monoclonal antibody, ngemva kokufukamela, uma ikhona babe I-BRU i-antibody, izohlangana ne-antigen eboshwe ngaphambili, i-antibody ekusampula block inhlanganisela ye-monoclonal antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha;Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula..
I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
1 |
| 1e/2e | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2 eya/4 | |
10 | i-serum dilution microplate | 1e/2e | |
11 | Isiyalezo | 1 ama-pcs |