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Ikhithi Yomkhuhlane we-Avian ELISA Kit Isithombe Esifakiwe
  • I-Avian Influenza Antibody ELISA Kit
  • I-Avian Influenza Antibody ELISA Kit

I-Avian Influenza Antibody ELISA Kit

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I-Avian Influenza Antibody ELISA Kit

Isifinyezo  Isetshenziselwa ukuthola amasosha omzimba athile ngokumelene ne-avian influenza virus (AIV) ku-serum
Isimiso

I-Avian influenza antibody Elisa kit isetshenziselwa ukuthola i-antibody ethize ngokumelene ne-Avian influenza Virus (AIV) ku-serum, ukuqapha i-antibody ngemva kwe-AIV yokuxilongwa kwe-immune kanye ne-serological yokutheleleka ku-Avian..
Okuhloswe ngakho Ukuthola I-Avian Influenza Antibody
Isampula I-Serum

 
Ubuningi 1 kit = 192 Test
 

 

Ukuzinza kanye Nesitoreji

1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi.

2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.

 

 

 

Ulwazi

I-Avian influenza, eyaziwa ngokungakahleleki ngokuthi i-avian flu noma i-bird flu, iyinhlobonhlobo yomkhuhlane obangelwa amagciwane ajwayelane nawo.izinyoni.
Uhlobo olunobungozi obukhulu kakhulu yi-pathogenic avian influenza (HPAI).Umkhuhlane wezinyoni uyafana neumkhuhlane wezingulube, umkhuhlane wenja, ihhashi
umkhuhlane kanye nomkhuhlane womuntu njengesifo esibangelwa amagciwane omkhuhlane ajwayele ukufana nokuthile
umsingathi.Ezinhlotsheni ezintathu zamagciwane omkhuhlane (A,B, futhiC), igciwane lomkhuhlane A liyi-i-zoonoticukutheleleka ngemvelo
i-reservoir cishe ngokuphelele ezinyoni.I-Avian influenza, ngezinjongo eziningi, ibhekisela egciwaneni lomkhuhlane A.

Isimiso Sokuhlolwa

Le kit isebenzisa block ELISA indlela, AIV antigen is pre-coated on microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululwe, ngemva kokufukamela, uma kukhona i-antibody ethize ye-AIV, izohlangana ne-antigen ehlanganiswe ngaphambili, ilahle i-antibody engahlanganisiwe nezinye izakhi ngokuwasha;bese wengeza i-enzyme ebizwa ngokuthi i-anti-AIV monoclonal antibody, i-antibody kusampula block inhlanganisela ye-monoclonal antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha.Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula.

Okuqukethwe

 

I-Reagent

Ivolumu

96 Izivivinyo/192Izivivinyo
1
I-microplate ehlanganiswe ne-antigen

 

1e/2e
2
 Ukulawula Okungalungile

 

2.0ml
3
 Ukulawula Okuhle

 

1.6ml
4
 Amasampula e-diluent

 

100ml
5
Isixazululo sokugeza (kugxilwe ku-10X)

 

100ml
6
 Ukuhlanganiswa kwe-enzyme

 

11/22ml
7
 I-substrate

 

11/22ml
8
 Isixazululo sokumisa

 

15ml
9
I-Adhesive plate sealer

 

2 eya/4
10 i-serum dilution microplate

1e/2e
11  Isiyalezo

1 ama-pcs

 

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