1.Faka i-reagent ku-100ml wamanzi wesampula, ngemva kokuqedwa, fukamela ku-36°C amahora angu-24.
2. Ukuhunyushwa kwemiphumela:
engenambala = negative
Okuphuzi = okuhle kuma-coliforms aphelele
Ophuzi + fluorescence = Escherichia coli positive.
QUANTlTATlVE DETECTlON
1. Faka ama-reagents kusampula yamanzi bese uxuba kahle
2. Thela kupuleti lokuthola inani lemithombo engu-51 (umthamo wepuleti lomthombo) noma ipuleti lokuthola inani lemithombo engu-97 (ipuleti lomthombo lomthamo)
3. Sebenzisa umshini wokuvala uphawu olawulwa wuhlelo
ukuvala i-quantitative discovery disc (umthamo wepuleti lomthombo) ukuze uvalwe futhi ufukamele ku-36°C amahora angu-24.
Isiko le-coliform elikwazi ukumelana nokushisa/i-fecal coliform elingu-44.5°C amahora angu-24 liphuzi futhi lilungile.
4. Ukuhunyushwa kwemiphumela:
engenambala = negative
I-checkered ephuzi = ama-coliform aphelele
Igridi ephuzi + ye-fluorescent = I-Escherichia coli ireferensi enhle yokubala kwetafula le-MPN