Isifinyezo | Ukutholwa kwe-Antibody ethize ye-Peste Des Petits Ruminants |
Isimiso | Ikhithi yokuhlola ye-PPRV ye-ELISA ukuze kutholwe amasosha omzimba e-Peste des petits ruminants virus ku-serum yezimvu nezimbuzi. |
Okuhloswe ngakho Ukuthola | I-antibody ye-PPRV |
Isampula | I-Serum
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Ubuningi | 1 kit = 192 Test |
Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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I-Ovine rinderpest, eyaziwa nangokuthipeste des petits ruminants(PPR), isifo esithathelwanayo esithinta ngokuyinhlokoizimbuzifuthiizimvu;nokho, amakamela nezincane zasendleezelusayokungathinteka futhi.I-PPR okwamanje ikhonaEnyakatho, Emaphakathi, EntshonalangafuthiEMpumalanga Afrika, iEmaphakathi Mpumalanga, futhiINingizimu Asia. Ibangelwaama-ruminants amancane e-morbillivirusohlotsheniI-Morbillivirus,futhi ihlobene eduze, phakathi kokunye, i-rinderpest morbillivirus, i-morbillivirus yesimungumungwane, futhii-canine morbillivirus(phambilini obewaziwa ngokuthiinkanii-distemper virus).Lesi sifo sithathelwana kakhulu, futhi singaba nezinga lokufa okungama-80-100%.acuteamacala kui-epizooticukulungiselelwa.Igciwane alitheleli abantu.
I-PPR yaziwa nangokuthi isifo sezimbuzi,ikati, i-syndrome ye-stomatitis-pneumoenteritis, kanye ne-ovine rinderpest.
Ama-ejensi asemthethweni afana ne-I-FAOfuthiI-OIEsebenzisa igama lesiFulentshi"peste des petits ruminants" ngezinhlobonhlobo zesipelingi.
Le kit isebenzisa indlela ye-ELISA yokuncintisana kuma-antigen e-PPRV ahlanganiswe ngaphambili emithonjeni ye-microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululiwe, ngemva kokufukamela, uma kukhona amasosha omzimba e-PPRV, izohlangana ne-antigen eboshwe ngaphambili, i-antibody eyisampula block inhlanganisela ye-monoclonal antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha;Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula.
I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
1 |
| 1e/2e | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2 eya/4 | |
10 | i-serum dilution microplate | 1e/2e | |
11 | Isiyalezo | 1 ama-pcs |