Imikhiqizo
I-Newcastle Disease Antibody ELISA Kit
I-Newcastle Disease Antibody ELISA Kit
| Isifinyezo | Ukutholwa kwe-Antibody ethize yezifo ezithile ze-Newcastle |
| Isimiso | Ikhithi ye-antibody ye-Newcastle Elisa isetshenziselwa ukuthola amasosha omzimba athile alwa nesifo saseNewcastle Igciwane (NDV) ku-serum, lokuqapha amasosha omzimba ngemva kwe-NDV immunekanye nokuxilongwa kwe-serological yokutheleleka ku-Avian.
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| Okuhloswe ngakho Ukuthola | I-antibody ye-Newcastle |
| Isampula | I-Serum
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| Ubuningi | 1 kit = 192 Test |
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Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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Ulwazi
Isifo saseNewcastle yisifo sezinyoni esithathelwanayo esihlasela izinhlobo eziningi zezinyoni zasekhaya nezasendle;liyasuleleka kubantu.Nakuba lingathelela abantu, izimo eziningi azinazo izimpawu;kuyaqabukela ingabangela umkhuhlane omncane kanye nezimpawu ezifana nomkhuhlane kanye/noma i-conjunctivitis kubantu.Imithelela yayo iphawuleka kakhulu ezinkukhuni ezifuywayo ngenxa yokuba lula kwazo kakhulu kanye namandla okuba nemithelela emibi ye-epizootic ezimbonini zezinkukhu.Itholakala emazweni amaningi.Akukho ukwelashwa kwaso okwaziwayo, kodwa ukusetshenziswa kwemijovo ye-prophylactic kanye nezindlela zokuhlanzeka kunciphisa amathuba okuqubuka kwezifo.
Isimiso Sokuhlolwa
Le kit isebenzisa indlela ye-ELISA, i-antigen ye-NDV ifakwe ngaphambili ku-microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululiwe, ngemva kokufukamela, uma kukhona i-antibody ethize ye-NDV, izohlangana ne-antigen ehlanganiswe ngaphambili, ilahle i-antibody engahlanganisiwe nezinye izakhi ngokuwasha;bese wengeza i-enzyme ebizwa ngokuthi i-anti-NDV monoclonal antibody, i-antibody kusampula block inhlanganisela ye-monoclonal antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha. Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula.
Okuqukethwe
| I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
| 1 |
| 1e/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2 eya/4 | |
| 10 | i-serum dilution microplate | 1e/2e | |
| 11 | Isiyalezo | 1 ama-pcs |
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