I-Anaplasma Phagocytophilum Ab Test Kit | |
Inombolo yekhathalogi | I-RC-CF26 |
Isifinyezo | Ukutholwa kwama-antibodies athile e-Anaplasmaphakathi nemizuzu eyi-10 |
Isimiso | Isinyathelo esisodwa sokuhlolwa kwe-immunochromatographic |
Okuhloswe ngakho Ukuthola | Ama-antibodies e-Anaplasma |
Isampula | Igazi le-Canine lonke, i-serum noma i-plasma |
Isikhathi sokufunda | 5 ~ 10 imizuzu |
Ukuzwela | 100.0 % vs. IFA |
Ukucaciswa | 100.0 % vs. IFA |
Umkhawulo Wokutholwa | I-IFA Titer 1/16 |
Ubuningi | Ibhokisi elingu-1 (ikhithi) = amadivaysi ayi-10 (Ukupakishwa komuntu ngamunye) |
Okuqukethwe | Ikhithi yokuhlola, ibhodlela le-Buffer, kanye nezinto ezilahlwayo |
Isexwayiso | Sebenzisa phakathi kwemizuzu eyi-10 ngemuva kokuvulaSebenzisa inani elifanele lesampula (0.01 ml ye-dropper) Sebenzisa ngemva kwemizuzu engu-15–30 e-RT uma egcinwe ngaphansi kwezimo ezibandayo Cabangela imiphumela yokuhlolwa njengengavumelekile ngemva kwemizuzu engu-10 |
Ibhaktheriya i-Anaplasma phagocytophilum (ngaphambilini eyayaziwa ngokuthi i-Ehrilichia phagocytophila) ingase ibangele ukutheleleka ezinhlobonhlobo zezilwane eziningana kuhlanganise nabantu.Lesi sifo esitholakala ezilwaneni ezifuywayo sibizwa nangokuthi i-tick-borne fever (TBF), futhi saziwa okungenani iminyaka engu-200.Amagciwane omndeni wakwa-Anaplasmataceae ane-gram-negative, nonmotile, coccoid to ellipsoid organisms, ahluka ngosayizi ukusuka ku-0.2 kuya ku-2.0um ububanzi.Ziyi-aerobes ephoqelekile, ayinayo indlela ye-glycolytic, futhi zonke ziyizilokazane ze-intracellular.Zonke izinhlobo zezinhlobo ze-Anaplasma zihlala kuma-vacuoles ane-membrane-lined kumaseli angakakhuli noma avuthiwe e-hematopoietic host host mammalian.I-phagocytophilum ihlasela ama-neutrophils kanti igama elithi granulocytotropic libhekisela kuma-neutrophils angenwe yileli gciwane.Izinto eziphilayo ezingavamile, ziye zatholakala kuma-eosinophils.
I-Anaplasma phagocytophilum
Izimpawu zomtholampilo ezivamile ze-canine anaplasmosis zihlanganisa ukushisa okukhulu, ukukhathala, ukucindezeleka kanye ne-polyarthritis.Izimpawu ze-neurologic (ataxia, seizures kanye nobuhlungu bentamo) nazo zingabonakala.Ukutheleleka kwe-Anaplasma phagocytophilum akuvamile ukubulawa ngaphandle uma kuyinkimbinkimbi ngezinye izifo.Ukulahlekelwa okuqondile, izimo ezikhubazekile kanye nokulahlekelwa kokukhiqiza kuye kwabonwa emazinyaneni.Ukukhipha isisu kanye ne-spermatogenesis ekhubazekile ezimvini nasezinkomeni kubhaliwe.Ubukhulu bokutheleleka buthonywa izici ezimbalwa, njengokuhlukahluka kwe-Anaplasma phagocytophilum ehilelekile, amanye amagciwane, ubudala, isimo sokuzivikela komzimba nesimo somsingathi, nezici ezifana nesimo sezulu nokuphathwa.Kufanele kukhulunywe ukuthi ukubonakaliswa komtholampilo kubantu kusuka ekuguleni okuncane okulinganiselwe okufana nomkhuhlane, kuya ekuthelelekeni okusongela ukuphila.Kodwa-ke, izifo eziningi zabantu cishe ziholela ekubonakalisweni komtholampilo okuncane noma kungabikho nhlobo.
I-Anaplasma phagocytophilum idluliselwa ngemikhaza ye-ixodid.E-United States ama-vector ayinhloko i-Ixodes scapularis ne-Ixodes pacificus, kuyilapho i-Ixode ricinus itholakale iyi-exophilic vector eyinhloko eYurophu.I-Anaplasma phagocytophilum isakazwa ngokudlulela yile mikhaza ye-vector, futhi abukho ubufakazi bokudluliselwa kwe-transovarial.Ucwaningo oluningi kuze kube manje oluphenye ngokubaluleka kwezilwane ezincelisayo ze-A. phagocytophilum kanye nezindawo ezithwala imikhaza zigxile kumagundane kodwa lesi silwane sinohlu olubanzi lwezilwane ezincelisayo, ezithelela amakati afuywayo, izinja, izimvu, izinkomo, namahhashi.
I-Indirect immunofluorescence assay ukuhlolwa okuyinhloko okusetshenziselwa ukuthola ukutheleleka.Amasampula e-serum yesigaba se-acute ne-convalescent angahlolwa ukuze kubhekwe ushintsho oluphindwe kane ku-antibody titer kuya ku-Anaplasma phagocytophilum.I-intracellular inclusions (morulea) ibonakala kuma-granulocyte ku-Wright noma ku-Gimsa enegazi elingcolisiwe.Izindlela ze-Polymerase chain reaction(PCR) zisetshenziselwa ukuthola i-Anaplasma phagocytophilum DNA.
Awukho umgomo otholakalayo wokuvikela ukutheleleka kwe-Anaplasma phagocytophilum.Ukuvimbela kuncike ekugwemeni ukuchayeka ku-vector yomkhaza (i-Ixodes scapularis, i-Ixodes pacificus, ne-Ixode ricinus) kusukela entwasahlobo kuya ekwindla, ukusetshenziswa kokuvimbela ama-antiacaricides, kanye nokusetshenziswa kwe-prophylactic ye-doxycycline noma i-tetracycline lapho uvakashela i-Ixodes scapularis, i-Ixodes pacificus, ne-Ixodes pacificus, ne-Ixode izifunda ezingapheli.