Isifinyezo | Ukutholwa okuqondileIsifo Sonyawo Nomlomo Uhlobo A amasosha omzimba |
Isimiso | Ikhithi yokuhlola ye-FMD yohlobo A lwe-ELISA ukuze kutholwe amasosha omzimba egciwane lesifo samatele ku-serum yezingulube, izinkomo, izimvu nezimbuzi ukuze kuhlolwe ukuzivikela komgomo we-FMD. |
Okuhloswe ngakho Ukuthola | Isifo Sonyawo Nomlomo Uhlobo A I-antibody |
Isampula | I-Serum
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Ubuningi | 1 kit = 192 Test |
Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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Igciwane lesifo sonyawo nomlomo(FMDV) yiyonai-pathogenlokho kubangelaisifo sonyawo nomlomo. Kuyi-apicornavirus, ilungu le-prototypical lohloboI-Aphthovirus.Lesi sifo, esidala ama-vesicles (amabhamuza) emlonyeni nasezinyaweniizinkomo, izingulube, izimvu, izimbuzi, nokunyeinselo ehlukeneizilwane ezithathelwanayo kakhulu futhi isifo esikhuluukufuywa kwezilwane.
Le khithi isebenzisa indlela ye-ELISA yokuncintisana kuma-antigen egciwane lesifo sonyawo nomlomo efakwe ngaphambili emithonjeni ye-microplate.Lapho uhlola, engeza isampula ye-serum ehlanjululiwe kanye ne-anti-FMD Ab, ngemva kokufukamela, uma kune-antibody ye-FMD, izohlangana ne-antigen eboshwe ngaphambili, i-antibody ekuyisampula block inhlanganisela ye-anti-FMD antibody kanye ne-antigen eboshwe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha;Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula.
Igciwane lesifo sonyawo nomlomokwenzeka ezinkulu eziyisikhombisaama-serotypes: O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1.Lawa ma-serotype akhombisa indawo ethile, futhi i-O serotype ivame kakhulu.
I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
1 |
| 1e/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2 eya/4 | |
10 | i-serum dilution microplate | 1e/2e | |
11 | Isiyalezo | 1 ama-pcs |