Imikhiqizo
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
| Isifinyezo | Use ukuthola i-antibody ethile ngokumelene ne-EDS76 ku-serum ngokufanelekile. |
| Isimiso | I-Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit isetshenziswa ukuthola i-antibody ethile ngokumelene ne-EDS76 ku-serum ngendlela efanele.Ukuze kuqashwe amasosha omzimba ngemva kwe-EDS76 yokuxilonga i-immune and serological of infection ku-Avian. |
| Okuhloswe ngakho Ukuthola | AI-antibody ngokumelene ne-EDS76 ku-serum |
| Isampula | I-Serum
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| Ubuningi | 1 kit = 192 Test |
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Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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Ulwazi
I-Egg Drop Syndrome (EDS-76) isifo esithathelwanayo esiyingozi esibangelwa i-adenovirus group III ye-Adenoviridae Avian Virus genus ene-hemagglutination.Kwamanye amapulazi ezinkukhu, ukukhiqizwa kwamaqanda amaningi ezinkukhu kwancipha kungazelelwe, futhi amaqanda akhubazekile njengamaqanda anegobolondo elithambile, amaqanda angenagobolondo namaqanda amagobolondo amancane akhiqizwa ngesikhathi esifanayo.Isikhathi sonke sesifo sihlala amasonto angu-5-6, emva kwalokho izinga lokukhiqiza amaqanda likhuphuka kancane kancane, kodwa kunzima ukufinyelela izinga ngaphambi kokuncipha.
Isimiso Sokuhlolwa
Le khithi isebenzisa indlela ye-ELISA engaqondile, i-antigen ehlanzekile ye-EDS76 igcotshwe ngaphambili emicupheni ye-enzyme encane.,uma kukhonaEDS76igciwane eliqondene nomuntu othize, lizohlangana ne-antigen elakhiwe ngaphambili, lilahle amasosha omzimba angahlanganisiwe nezinye izakhi ngokuwasha;bese wengeza i-enzyme ebizwa ngokuthi i-anti-EDS76 virus monoclonal antibody, bese uhlanganisa i-monoclonal antibody kanye ne-antigen ehlanganiswe ngaphambili;lahla i-enzyme engahlanganisiwe ehlangene ngokuwasha;Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis isengxenyeni ephambene yokuqukethwe kwamasosha omzimba kusampula, sebenzisa isifundi se-ELISA ku-450nm wavelenth ukuze ulinganise ukumunca A inani emithonjeni yokusabela ngemva kokwengeza isisombululo sokumisa.
ukumisa ukusabela.
Okuqukethwe
| I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
| 1 |
| 1e/2e | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2 eya/4 | |
| 10 | i-serum dilution microplate | 1e/2e | |
| 11 | Isiyalezo | 1 ama-pcs |
