Isifinyezo | Ukutholwa kwe-antibody ethile ye-Bovine Tuberculosis(BTB). |
Isimiso | Ikhithi yokuhlola ye-Bovine Tuberculosis(BTB) Elisa ingase isetshenziselwe ukuthola i-Bovine Tuberculosis ku-serum noma ku-plasma ye-Bovine. |
Okuhloswe ngakho Ukuthola | I-antibody ye-Bovine Tuberculosis (BTB). |
Isampula | I-Serum
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Ubuningi | 1 kit = 192 Test |
Ukuzinza kanye Nesitoreji | 1) Wonke ama-reagents kufanele agcinwe ku-2 ~ 8℃.Ungaqandi. 2) Impilo yeshelufu yizinyanga eziyi-12.Sebenzisa wonke ama-reagents ngaphambi kosuku lokuphelelwa yisikhathi kukhithi.
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I-Mycobacterium bovis ikhula kancane (isikhathi sokuzala kwamahora angu-16- kuya kwangu-20) i-aerobic bacterium kanye ne-causative agent yesifo sofuba ezinkomeni (eyaziwa ngokuthi i-TB yezinkomo).Ihlobene ne-Mycobacterium tuberculosis, ibhaktheriya ebangela isifo sofuba kubantu.I-M. bovis ingagxuma umgoqo wezinhlobo zezilwane futhi ibangele ukutheleleka okufana nesifo sofuba kubantu nakwezinye izilwane ezincelisayo.
Lokhu ikhithi sebenzisa ngokungaqondile ELISA indlela, okuhlanzekile I-BTB i-antigen is efakwe ngaphambili on i-enzyme kahle kahle imichilo. Uma uhlola, engeza buhlanjululiwe i-serum isampula, ngemva ukufukamela, if Lapho is I-BTB ethize amasosha omzimba, it intando hlanganisa nge i efakwe ngaphambili i-antigen, lahla i engahlanganisiwe amasosha omzimba futhi okunye izingxenye nge ukuwasha; bese engeza i-enzyme hlanganisa, lahla i engahlanganisiwe i-enzyme hlanganisa nge
ukuwasha. Engeza i-TMB substrate emithonjeni emincane, isignali eluhlaza okwesibhakabhaka nge-Enzyme catalysis iqondile ingxenye yokuqukethwe kwamasosha omzimba kusampula.
I-Reagent | Ivolumu 96 Izivivinyo/192Izivivinyo | ||
1 |
| 1e/2e | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2 eya/4 | |
10 | i-serum dilution microplate | 1e/2e | |
11 | Isiyalezo | 1 ama-pcs |